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KMID : 0363620120330030133
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Journal of Korean Oriental Medicine
2012 Volume.33 No. 3 p.133 ~ p.146
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Effects of YideungJetong-Tang on Peripheral Neuropathy Induced by Taxol and Compression Injury in the Rat Sciatic Nerve
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Jung Ho-Young
Kim Chul-Jung
Cho Chung-Sik
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Abstract
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Background: Most antitumor agents have the side effect of chemotherapy-induced peripheral neuropathy (CIPN).
Cancer patients who take antitumor agents suffer from CIPN, but there is no known treatment for it. Unlike the
central nerve system, the peripheral nerve can self-repair, and the Schwann cell takes this mechanism.
Objectives: In this study, we researched the effect of YideungJetong-Tang (YJT) extract on taxol-induced sciatic nerve
damage, through in vitro and in vivo experiments. Also, we studied the effect of YJT extract on neurite recovery
and anti-inflammatory effect after compression injury of sciatic nerve in vivo.
Methods: Vehicle, taxol and taxol+YJT were respectively applied on sciatic nerve cells of rat in vitro, then the cells
were cultured. The sciatic nerve cells and Schwann cells were then observed using Neurofilament 200, Hoechst, β
-tubulin, S-100β, caspase-3 and phospho-Erk1/2.
CIPN was induced by taxol into the sciatic nerve of rat in vivo, then YJT extract was taken orally. The axons,
Schwann cells and neurites of the DRG sensory nerve were then observed using Neurofilament 200, β-tubulin,
Hoechst, S-100β, phospho-Erk1/2 and caspase-3.
YJT was taken orally after sciatic nerve compression injury, and the changes in axon of the sciatic nerve, Schwann
cells and TNF-α concentration were observed.
Results: The taxol and YJT treated group showed significant effects on Schwann cell recovery, neurite growth and
recovery. In vivo, YJT compared with control group showed Schwann cell structural improvement and axons
recovering effect after taxol-induced Schwann cell damage. After sciatic nerve compression injury, recovery of distal
axon, changes of Schwann cell distribution, and anti-inflammatory response were observed in the YJT.
Conclusions: Through this study, we found that after taxol-induced neurite damage of sciatic nerve in vivo and in
vitro, YJT had significant effects on sciatic nerve growth and Schwann cell structural improvement. In vivo, YJT
improved recovery of distal axons and Schwann cells and had an anti-inflammatory effect.
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KEYWORD
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Taxol , YideungJetong-Tang (YJT) , sciatic nerve , Schwann cell , TNF-α
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